Kansas Water Science Center
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Detection of Herbicides and Herbicide Metabolites Using Cross Reactivity of Enzyme ImmunoassaysBy E.M. ThurmanAbstractEnzyme immunoassay is an important environmental analysis method that may identify many herbicide analytes in water samples. Because of similarities in chemical structure among various members of a herbicide class, there often may be an unwanted response that is characterized by a percentage of cross reactivity. There may also be cross reactivity caused by degradation products of the target analyte that are present in the sample. In this paper, the concept of cross reactivity caused by degradation products or by nontarget analytes is explored as a tool for identification of degradation products or structurally similar compounds previously not known to be present. Two examples are examined from various water-quality studies. The are alachlor and its metabolite, alachlor ethane sulfonic acid, and atrazine and its class members, prometryn and propazine. The use of cross reactivity to initally detect these compound is explained. Additional information about the Organic Geochemistry Research Laboratory can be found at: http://ks.water.usgs.gov/studies/reslab/Thurman, E.M., 2000, Detection of herbicides and herbicide metabolites using cross reactivity of enzyme immunoassays [abst.]: Pacifichem 2000, Honolulu, Hawaii, Debember 14-19, 2000. |
U.S. Department of the Interior |
U.S. Geological Survey
URL: /pubs/abstracts/emt.1200.html
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The USGS has collected hydrologic information in Kansas since 1895 with the first USGS Cooperative
Water Program funding agreement for 6 streamflow gages with the Kansas Water Board. USGS collects streamflow
and gage-height data; reservoir content; water-quality and water-quantity data; suspended-sediment data;
and groundwater levels. Hydrologic studies are conducted on national, regional, statewide, and local levels.
The USGS in Kansas works cooperatively with 31 Federal, State, and local agencies, such as the Kansas Water Office, the U.S.Army Corps of Engineers, and the City of Wichita