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earth GRL

Organic Geochemistry Research Laboratory
Enzyme Linked-Imunnosorbent Assay


Immunoassays are rapid screening techinques that can be used to provide data on a specific compound or class of compunds on a large number of samples. We have used these methods to conduct large scale reconnaissance studies of atrazine in rainfall and cyanobacterial toxins in the USEPA national lake and reservoir reconnaissance study.


  • Sample collection requirements:
    • IMA, IMB: 2-125mL (4 oz) amber glass bottles with teflon lined caps per sample.
    • All other ELISAs should be collected in plastic bottles. Send either 250mL (8oz) or 500mL (16oz) plain poly bottles.
    • For samples being submitted for total analysis (IMNT, IMYT, and IMXT) only fill the bottles half-way to ensure that they do not break when frozen.
  • Instrumentation: Enzyme-Linked immunosorbent assay (ELISA)

Compound Name Reporting Limit Parameter Code Method Code CAS #
Atrazine (IMA) 0.10 μg/L 99775 IMM01 1912249
17 beta-estradiol (IMB) 2.50 ng/L 89002 IMM04 50-28-2
Total microcystin/nodularians (IMNT) 0.10 μg/L 89011 IMM06 101043-37-2
Dissolved microcystin/nodularians (IMND) 0.10 μg/L 89012 IMM07 101043-37-2
Total saxitoxin (IMXT) 0.02 μg/L 89003 IMM08 35523-89-8
Dissolved saxitoxin (IMXD) 0.02 μg/L 89004 IMM09 35523-89-8
Total cylindrospermopsin (IMTY) 0.05 μg/L 89005 IMM10 143545-90-8
Dissolved cylindrospermopsin (IMYD) 0.05 μg/L 89006 IMM11 143545-90-8

Publications:

2012 1995 1991
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